Synaptic Systems

Cat. No.: 310 111

Amount: 100 µg

Price: $420.00

Cat. No. 310 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (ECL detection) gallery IP: yes ICC: yes (see remarks) gallery IHC: not tested yet IHC-P: 1 : 200 up to 1 : 1000 gallery
Clone	330G5
Subtype	IgG2a (κ light chain)
Immunogen	Full-length recombinant human MLC-2V protein (UniProt Id: P10916)
Epitop	AA 105 to 111 from human MLC-2V (UniProt Id: P10916)
Reactivity	Reacts with: human (P10916), rat (P08733), mouse (P51667), pig, chicken. Other species not tested yet.
Specificity	Specific for MLC-2V, no cross-reactivity to MLC-2A.
Remarks	ICC: Methanol fixation gives stronger signals.
Data sheet	310_111.pdf

References for MLC-2V - 310 111

WB (1)
IP (1)
ICC (13)
IHC (6)
IHC-P (3)
FACS (3)

Creatine uptake in mouse hearts with genetically altered creatine levels.
ten Hove M, Makinen K, Sebag-Montefiore L, Hunyor I, Fischer A, Wallis J, Isbrandt D, Lygate C, Neubauer S
Journal of molecular and cellular cardiology (2008) 453: 453-9. 310 111 WB

Transformation of the Nonprocessive Fast Skeletal Myosin II into a Processive Motor.
Amrute-Nayak M, Nayak A, Steffen W, Tsiavaliaris G, Scholz T, Brenner B
Small (Weinheim an der Bergstrasse, Germany) (2019) : e1804313. 310 111 IP

Prospective Isolation of ISL1+Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy.
Ghazizadeh Z, Fattahi F, Mirzaei M, Bayersaikhan D, Lee J, Chae S, Hwang D, Byun K, Tabar MS, Taleahmad S, Mirshahvaladi S, et al.
Stem cell reports (2018) 103: 848-859. 310 111 ICC; tested species: human

Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells.
Baljinnyam E, Venkatesh S, Gordan R, Mareedu S, Zhang J, Xie LH, Azzam EI, Suzuki CK, Fraidenraich D
Physiological reports (2017) 515: . 310 111 ICC; tested species: human

Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 310 111 ICC

Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions.
Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, Kojima H, Umei T, Akiyama M, Kuishi Y, Kurokawa J, et al.
Stem cell reports (2015) 56: 1128-1142. 310 111 ICC

Phasic modulation of Wnt signaling enhances cardiac differentiation in human pluripotent stem cells by recapitulating developmental ontogeny.
Mehta A, Ramachandra CJ, Sequiera GL, Sudibyo Y, Nandihalli M, Yong PJ, Koh CH, Shim W
Biochimica et biophysica acta (2014) 184311: 2394-402. 310 111 ICC

Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Jung CB, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, Dorn T, Ruppenthal S, Pfeiffer S, Goedel A, Dirschinger RJ, et al.
EMBO molecular medicine (2012) 43: 180-91. 310 111 ICC; tested species: human

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, et al.
Nature methods (2011) 812: 1037-40. 310 111 ICC

Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos.
Herrmann F, Bundschu K, Kühl SJ, Kühl M
Developmental dynamics : an official publication of the American Association of Anatomists (2011) 24012: 2634-45. 310 111 ICC

c-MYC independent nuclear reprogramming favors cardiogenic potential of induced pluripotent stem cells.
Martinez-Fernandez A, Nelson TJ, Ikeda Y, Terzic A
Journal of cardiovascular translational research (2010) 31: 13-23. 310 111 ICC

Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression.
Rust W, Balakrishnan T, Zweigerdt R
Regenerative medicine (2009) 42: 225-37. 310 111 ICC

Diastolic dysfunction in alveolar hypoxia: a role for interleukin-18-mediated increase in protein phosphatase 2A.
Larsen KO, Lygren B, Sjaastad I, Krobert KA, Arnkvaern K, Florholmen G, Larsen AK, Levy FO, Taskén K, Skjønsberg OH, Christensen G, et al.
Cardiovascular research (2008) 801: 47-54. 310 111 ICC

Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 310 111 ICC

Cardiomyocytes from human and mouse embryonic stem cells.
Mummery C, van der Heyden MA, de Boer TP, Passier R, Ward D, van den Brink S, van Rooijen M, van de Stolpe A
Methods in molecular medicine (2007) 140: 249-72. 310 111 ICC; tested species: human,mouse

Recapitulation of dyssynchrony-associated contractile impairment in asymmetrically paced engineered heart tissue.
Stenzig J, Lemoine MD, Stoter AMS, Wrona KM, Lemme M, Mulla W, Etzion Y, Eschenhagen T, Hirt MN
Journal of molecular and cellular cardiology (2022) 163: 97-105. 310 111 IHC; tested species: human

Hypertrophic signaling compensates for contractile and metabolic consequences of DNA methyltransferase 3A loss in human cardiomyocytes.
Madsen A, Krause J, Höppner G, Hirt MN, Tan WLW, Lim I, Hansen A, Nikolaev VO, Foo RSY, Eschenhagen T, Stenzig J, et al.
Journal of molecular and cellular cardiology (2021) 154: 115-123. 310 111 IHC; tested species: human

An Important Role for DNMT3A-Mediated DNA Methylation in Cardiomyocyte Metabolism and Contractility.
Madsen A, Höppner G, Krause J, Hirt MN, Laufer SD, Schweizer M, Tan WLW, Mosqueira D, Anene-Nzelu CG, Lim I, Foo RSY, et al.
Circulation (2020) : . 310 111 IHC; tested species: human

Effects of the Delta Opioid Receptor Agonist DADLE in a Novel Hypoxia-Reoxygenation Model on Human and Rat-Engineered Heart Tissue: A Pilot Study.
Funcke S, Werner TR, Hein M, Ulmer BM, Hansen A, Eschenhagen T, Hirt MN
Biomolecules (2020) 109: . 310 111 IHC; tested species: rat

Guided cardiopoiesis enhances therapeutic benefit of bone marrow human mesenchymal stem cells in chronic myocardial infarction.
Behfar A, Yamada S, Crespo-Diaz R, Nesbitt JJ, Rowe LA, Perez-Terzic C, Gaussin V, Homsy C, Bartunek J, Terzic A
Journal of the American College of Cardiology (2010) 569: 721-34. 310 111 IHC

Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction.
van Laake LW, Passier R, Monshouwer-Kloots J, Verkleij AJ, Lips DJ, Freund C, den Ouden K, Ward-van Oostwaard D, Korving J, Tertoolen LG, van Echteld CJ, et al.
Stem cell research (2007) 11: 9-24. 310 111 IHC

Cardiotoxicity assessment using 3D vascularized cardiac tissue consisting of human iPSC-derived cardiomyocytes and fibroblasts.
Tadano K, Miyagawa S, Takeda M, Tsukamoto Y, Kazusa K, Takamatsu K, Akashi M, Sawa Y
Molecular therapy. Methods & clinical development (2021) 22: 338-349. 310 111 IHC-P; tested species: human

Rat atrial engineered heart tissue: a new in vitro model to study atrial biology.
Krause J, Löser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T, Stenzig J
Basic research in cardiology (2018) 1135: 41. 310 111 IHC-P; tested species: rat

p38 MAPK-dependent small HSP27 and αB-crystallin phosphorylation in regulation of myocardial function following cardioplegic arrest.
Clements RT, Feng J, Cordeiro B, Bianchi C, Sellke FW
American journal of physiology. Heart and circulatory physiology (2011) 3005: H1669-77. 310 111 IHC-P; tested species: rat

A Multiwell Cardiac μGMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs.
Edwards SL, Zlochiver V, Conrad DB, Vaidyanathan R, Valiquette AM, Joshi-Mukherjee R
Stem cell reports (2018) : . 310 111 FACS; tested species: human

Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, Christalla P, Malan D, Hesse M, Bosio A, Fleischmann BK, et al.
PloS one (2015) 1011: e0143538. 310 111 FACS

Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling.
Iglesias-García O, Pelacho B, Prósper F
Journal of molecular and cellular cardiology (2013) 62: 43-50. 310 111 FACS; tested species: human

Cat. No.: 310 111

Amount: 100 µg

Price: $420.00

References
WB (1)
IP (1)
ICC (13)
IHC (6)
IHC-P (3)
FACS (3)

Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression.
Rust W, Balakrishnan T, Zweigerdt R
Regenerative medicine (2009) 42: 225-37. 310 111 ICC

ICC

IHC-P

Background

During cardiogenesis two major isoforms of myosin light chain 2 are co-expressed in a tightly regulated manner. MLC-2V is only present in the ventricle while MLC-2A is exclusively expressed in the atrium. Knock out studies revealed that the 2A isoform cannot substitute for the 2V variant in the ventricular chamber.
Recently it has been demonstrated that embryonic and adult stem cells can be differentiated into cardiomyocytes which may generate suitable replacements for damaged heart tissue in the future.
These antibodies are useful tools to distinguish between ventricle and atrium specific cardiomyocytes.

1 ) Satin J et al., J. Physiol. (Lond.) (2004)
2 ) Müller M et al., FASEB J. (2000)
3 ) Palermo J et al., Circ. Res. (1996)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Methanol fixation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

MLC-2V antibody - 310 111

References for MLC-2V - 310 111